| Transactions |
| Date | Team | Player | Type | OthTm | EffDate | When |
| 4/12/1965 | Min | Allen | Placed on disabled list |   |   | Before |
| 4/16/1965 | Hou | Hoffman | Demoted to reserve roster |   |   | Before |
| 4/16/1965 | Hou | Jackson | Demoted to reserve roster |   |   | Before |
| 4/16/1965 | Mil | Olivo | Promoted to active roster |   |   | Before |
| 4/22/1965 | NYN | Collins | Placed on disabled list |   |   | Before |
| 4/24/1965 | Bal | Larsen | Acquired via trade | Hou |   | Before |
| 4/24/1965 | Hou | Larsen | Traded away | Bal |   | Before |
| 4/27/1965 | NYN | Berra | Signed |   |   | Before |
| 4/27/1965 | Was | Hannan | Demoted to reserve roster |   |   | Before |
| 4/28/1965 | Cal | Wantz | Placed on disabled list |   |   | Before |
| 5/2/1965 | Det | Rakow | Promoted to active roster |   |   | After |
| 5/2/1965 | Det | Seale | Demoted to reserve roster |   |   | After |
| 5/3/1965 | KC | Blanchard | Acquired via trade | NYA |   | Before |
| 5/3/1965 | KC | Edwards | Traded away | NYA |   | Before |
| 5/3/1965 | KC | Sheldon | Acquired via trade | NYA |   | Before |
| 5/3/1965 | LA | Davis,T | Placed on disabled list |   |   | Before |
| 5/3/1965 | LA | Griffith | Promoted to active roster |   |   | Before |
| 5/3/1965 | LA | Johnson | Promoted to active roster |   |   | Before |
| 5/3/1965 | LA | Oliver | Demoted to reserve roster |   |   | Before |
| 5/3/1965 | LA | Smith | Demoted to reserve roster |   |   | Before |
| 5/3/1965 | NYA | Blanchard | Traded away | KC |   | Before |
| 5/3/1965 | NYA | Edwards | Acquired via trade | KC |   | Before |
| 5/3/1965 | NYA | Howard | Placed on disabled list |   |   | Before |
| 5/3/1965 | NYA | Sheldon | Traded away | KC |   | Before |
| 5/4/1965 | Cin | Duffalo | Acquired via trade | SF |   | Before |
| 5/4/1965 | Cin | Henry | Traded away | SF |   | Before |
| 5/4/1965 | Cle | Salmon | Promoted to active roster |   |   | Before |
| 5/4/1965 | SF | Duffalo | Traded away | Cin |   | Before |
| 5/4/1965 | SF | Henry | Acquired via trade | Cin |   | Before |
| 5/4/1965 | SF | Murakami | Promoted to active roster |   |   | Before |
| 5/5/1965 | Bal | Knowles | Demoted to reserve roster |   |   | Before |
| 5/5/1965 | ChN | Faul | Demoted to reserve roster |   |   | Before |
| 5/5/1965 | KC | Reynolds | Demoted to reserve roster |   |   | Before |
| 5/5/1965 | KC | Santiago | Demoted to reserve roster |   |   | Before |
| 5/5/1965 | StL | Spiezio | Demoted to reserve roster |   |   | Before |
| 5/7/1965 | Bos | Schlesinger | Waived | KC |   | Before |
| 5/7/1965 | SF | Cepeda | Placed on disabled list |   |   | Before |
| 5/10/1965 | Cle | Barker | Traded away | NYA |   | Before |
| 5/10/1965 | Cle | Gonzalez | Acquired via trade | NYA |   | Before |
| 5/10/1965 | NYA | Barker | Acquired via trade | Cle |   | Before |
| 5/10/1965 | NYA | Carmel | Demoted to reserve roster |   |   | Before |
| 5/10/1965 | NYA | Clarke | Promoted to active roster |   |   | Before |
| 5/10/1965 | NYA | Gonzalez | Traded away | Cle |   | Before |
| 5/11/1965 | NYN | Hiller | Acquired via trade | SF |   | Before |
| 5/11/1965 | Pit | Face | Placed on disabled list |   |   | Before |
| 5/11/1965 | SF | Hiller | Traded away | NYN |   | Before |
| 5/11/1965 | SF | Priddy | Demoted to reserve roster |   |   | Before |
| 5/11/1965 | Was | Green | Traded away | Phi |   | Before |
| 5/11/1965 | Was | Willhite | Traded away | LA |   | Before |
| 5/12/1965 | Bal | Rowe | Demoted to reserve roster |   |   | Before |
| 5/12/1965 | Bal | Starrette | Demoted to reserve roster |   |   | Before |
| 5/12/1965 | Bos | Nixon | Demoted to reserve roster |   |   | Before |
| 5/12/1965 | ChN | Baumann | Demoted to reserve roster |   |   | Before |
| 5/12/1965 | Cin | Boros | Demoted to reserve roster |   |   | Before |
| 5/12/1965 | Cin | Helms | Demoted to reserve roster |   |   | Before |
| 5/12/1965 | Cle | Scheinblum | Demoted to reserve roster |   |   | Before |
| 5/12/1965 | Cle | Williams | Demoted to reserve roster |   |   | Before |
| 5/12/1965 | Det | Navarro | Demoted to reserve roster |   |   | Before |
| 5/12/1965 | Det | Roman | Demoted to reserve roster |   |   | Before |
| 5/12/1965 | Det | Sullivan | Demoted to reserve roster |   |   | Before |
| 5/12/1965 | Hou | Fox | Signed |   |   | Before |
| 5/12/1965 | Hou | White | Demoted to reserve roster |   |   | Before |
| 5/12/1965 | KC | Sanders | Demoted to reserve roster |   |   | Before |
| 5/12/1965 | Mil | Aaron,T | Demoted to reserve roster |   |   | Before |
| 5/12/1965 | Mil | Olivo | Demoted to reserve roster |   |   | Before |
| 5/12/1965 | Min | Reese | Demoted to reserve roster |   |   | Before |
| 5/12/1965 | Min | Tovar | Demoted to reserve roster |   |   | Before |
| 5/12/1965 | NYN | Jones | Demoted to reserve roster |   |   | Before |
| 5/12/1965 | NYN | Willey | Demoted to reserve roster |   |   | Before |
| 5/12/1965 | Phi | Corrales | Demoted to reserve roster |   |   | Before |
| 5/12/1965 | Phi | Del Greco | Demoted to reserve roster |   |   | Before |
| 5/12/1965 | Phi | Steevens | Demoted to reserve roster |   |   | Before |
| 5/12/1965 | Was | Bridges | Promoted to active roster |   |   | Before |
| 5/13/1965 | Mil | Menke | Placed on disabled list |   |   | After |
| 5/13/1965 | Was | Duckworth | Demoted to reserve roster |   |   | After |
| 5/14/1965 | Cal | Cimoli | Released |   |   | Before |
| 5/14/1965 | Mil | Woodward | Promoted to active roster |   |   | Before |
| 5/15/1965 | Was | Sievers | Released |   |   | Before |
| 5/16/1965 | SF | Hands | Demoted to reserve roster |   |   | Before |
| 5/17/1965 | NYN | Berra | Released |   |   | Before |
| 5/18/1965 | Cle | Martinez | Demoted to reserve roster |   |   | Before |
| 5/19/1965 | Cal | Piersall | Placed on disabled list |   |   | Before |
| 5/19/1965 | Cal | Smith,BG | Promoted to active roster |   |   | Before |
| 5/19/1965 | Det | Hamilton | Promoted to active roster |   |   | Before |
| 5/19/1965 | Det | Nischwitz | Promoted to active roster |   |   | Before |
| 5/19/1965 | Det | Rakow | Demoted to reserve roster |   |   | Before |
| 5/19/1965 | Det | Smith | Demoted to reserve roster |   |   | Before |
| 5/19/1965 | Was | Koplitz | Promoted to active roster |   |   | Before |
| 5/19/1965 | Was | Moore | Promoted to active roster |   |   | Before |
| 5/22/1965 | Pit | Pagan | Acquired via trade | SF |   | After |
| 5/22/1965 | Pit | Schofield | Traded away | SF |   | After |
| 5/22/1965 | SF | Pagan | Traded away | Pit |   | After |
| 5/22/1965 | SF | Schofield | Acquired via trade | Pit |   | After |
| 5/23/1965 | Hou | Beauchamp | Traded away | Mil |   | After |
| 5/23/1965 | Hou | Johnson | Traded away | Mil |   | After |
| 5/23/1965 | Hou | Maye | Acquired via trade | Mil |   | After |
| 5/23/1965 | Mil | Beauchamp | Acquired via trade | Hou |   | After |
| 5/23/1965 | Mil | Beauchamp | Demoted to reserve roster |   |   | After |
| 5/23/1965 | Mil | Johnson | Acquired via trade | Hou |   | After |
| 5/23/1965 | Mil | Maye | Traded away | Hou |   | After |
| 5/24/1965 | Pit | Butters | Placed on disabled list |   |   | Before |
| 5/25/1965 | ChA | Agee | Demoted to reserve roster |   |   | Before |
| 5/25/1965 | Pit | Carpin | Promoted to active roster |   |   | Before |
| 5/25/1965 | Was | McCabe | Demoted to reserve roster |   |   | Before |
| 5/26/1965 | Bos | Duliba | Promoted to active roster |   |   | Before |
| 5/26/1965 | Bos | Heffner | Demoted to reserve roster |   |   | Before |
| 5/26/1965 | NYN | Miller | Promoted to active roster |   |   | Before |
| 5/28/1965 | KC | Harrison | Traded away | Was |   | Before |
| 5/28/1965 | KC | Mossi | Promoted to active roster |   |   | Before |
| 5/28/1965 | LA | Gilliam | Promoted to active roster |   |   | Before |
| 5/28/1965 | LA | Griffith | Demoted to reserve roster |   |   | Before |
| 5/29/1965 | ChN | Bailey | Acquired via trade | SF |   | After |
| 5/29/1965 | ChN | Bertell | Traded away | SF |   | After |
| 5/29/1965 | ChN | Burke | Demoted to reserve roster |   |   | After |
| 5/29/1965 | ChN | Gabrielson | Traded away | SF |   | After |
| 5/29/1965 | ChN | Hendley | Acquired via trade | SF |   | After |
| 5/29/1965 | ChN | Krug | Promoted to active roster |   |   | After |
| 5/29/1965 | ChN | Kuenn | Acquired via trade | SF |   | After |
| 5/29/1965 | Hou | Kasko | Placed on disabled list |   |   | Before |
| 5/29/1965 | SF | Bailey | Traded away | ChN |   | After |
| 5/29/1965 | SF | Bertell | Acquired via trade | ChN |   | After |
| 5/29/1965 | SF | Gabrielson | Acquired via trade | ChN |   | After |
| 5/29/1965 | SF | Hands | Promoted to active roster |   |   | After |
| 5/29/1965 | SF | Hendley | Traded away | ChN |   | After |
| 5/29/1965 | SF | Kuenn | Traded away | ChN |   | After |
| 5/30/1965 | ChN | Burdette | Traded away | Phi |   | Before |
| 5/30/1965 | Hou | Mahoney | Promoted to active roster |   |   | Before |
| 5/30/1965 | Phi | Burdette | Acquired via trade | ChN |   | Before |
| 6/1/1965 | Cal | Lee,D | Traded away | Hou |   | Before |
| 6/1/1965 | Cal | Spangler | Acquired via trade | Hou |   | Before |
| 6/1/1965 | Hou | Lee | Acquired via trade | Cal |   | Before |
| 6/1/1965 | Hou | Spangler | Traded away | Cal |   | Before |
| 6/1/1965 | Phi | Duren | Released |   |   | Before |
| 6/1/1965 | StL | Ricketts | Demoted to reserve roster |   |   | Before |
| 6/2/1965 | Mil | Kelley | Promoted to active roster |   |   | Before |
| 6/2/1965 | NYN | Ribant | Demoted to reserve roster |   |   | Before |
| 6/4/1965 | Hou | Gentile | Acquired via trade | KC |   | Before |
| 6/4/1965 | KC | Gentile | Traded away | Hou |   | Before |
| 6/4/1965 | KC | Hickman | Promoted to active roster |   |   | Before |
| 6/4/1965 | Min | Allen | Activated from disabled list |   |   | After |
| 6/4/1965 | NYA | Howard | Activated from disabled list |   |   | Before |
| 6/5/1965 | Min | Allen | Demoted to reserve roster |   |   | Before |
| 6/6/1965 | LA | Brewer | Placed on disabled list |   |   | After |
| 6/7/1965 | Bos | Horton | Promoted to active roster |   |   | Before |
| 6/8/1965 | Mil | Carroll | Demoted to reserve roster |   |   | Before |
| 6/8/1965 | Mil | Tiefenauer | Demoted to reserve roster |   |   | Before |
| 6/8/1965 | NYA | Lopez,A | Demoted to reserve roster |   |   | Before |
| 6/8/1965 | Was | Duren | Signed |   |   | Before |
| 6/11/1965 | ChN | Kessinger | Promoted to active roster |   |   | Before |
| 6/11/1965 | ChN | Pena | Demoted to reserve roster |   |   | Before |
| 6/12/1965 | LA | Willhite | Promoted to active roster |   |   | Before |
| 6/15/1965 | Cal | Piche | Demoted to reserve roster |   |   | Before |
| 6/15/1965 | Cal | Roof | Traded away | Cle |   | Before |
| 6/15/1965 | Cal | Satriano | Promoted to active roster |   |   | Before |
| 6/15/1965 | Cal | Shockley | Demoted to reserve roster |   |   | Before |
| 6/15/1965 | Cle | Carreon | Demoted to reserve roster |   |   | Before |
| 6/15/1965 | Cle | Donovan | Released |   |   | Before |
| 6/15/1965 | Cle | Moran | Demoted to reserve roster |   |   | Before |
| 6/15/1965 | Cle | Roof | Acquired via trade | Cal |   | Before |
| 6/15/1965 | Cle | Sims | Promoted to active roster |   |   | Before |
| 6/15/1965 | Cle | Spring | Promoted to active roster |   |   | Before |
| 6/15/1965 | Hou | Bateman | Demoted to reserve roster |   |   | Before |
| 6/15/1965 | Hou | Cuellar | Promoted to active roster |   |   | Before |
| 6/15/1965 | Hou | Taylor | Acquired via trade | StL |   | Before |
| 6/15/1965 | Hou | Triandos | Acquired via trade | Phi |   | Before |
| 6/15/1965 | Hou | Woodeshick | Traded away | StL |   | Before |
| 6/15/1965 | KC | Drabowsky | Demoted to reserve roster |   |   | Before |
| 6/15/1965 | KC | Stahl | Promoted to active roster |   |   | Before |
| 6/15/1965 | NYA | Gibbs | Promoted to active roster |   |   | Before |
| 6/15/1965 | NYA | Mikkelsen | Demoted to reserve roster |   |   | Before |
| 6/15/1965 | NYA | Schmidt | Demoted to reserve roster |   |   | Before |
| 6/15/1965 | NYN | Taylor | Demoted to reserve roster |   |   | Before |
| 6/15/1965 | Phi | Corrales | Promoted to active roster |   |   | Before |
| 6/15/1965 | Phi | Triandos | Traded away | Hou |   | Before |
| 6/15/1965 | SF | Hands | Demoted to reserve roster |   |   | Before |
| 6/15/1965 | StL | Taylor | Traded away | Hou |   | Before |
| 6/15/1965 | StL | Woodeshick | Acquired via trade | Hou |   | Before |
| 6/16/1965 | Bal | Miller,J | Promoted to active roster |   |   | Before |
| 6/16/1965 | Bos | Nixon | Promoted to active roster |   |   | Before |
| 6/16/1965 | Cal | Ranew | Promoted to active roster |   |   | Before |
| 6/16/1965 | Mil | Tiefenauer | Traded away | NYA |   | Before |
| 6/16/1965 | Min | Allen | Promoted to active roster |   |   | Before |
| 6/16/1965 | NYA | Tiefenauer | Acquired via trade | Mil |   | Before |
| 6/16/1965 | NYN | Stephenson | Promoted to active roster |   |   | Before |
| 6/17/1965 | Mil | Menke | Activated from disabled list |   |   | Before |
| 6/17/1965 | StL | Dennis | Promoted to active roster |   |   | After |
| 6/17/1965 | StL | Javier | Placed on disabled list |   |   | After |
| 6/22/1965 | Hou | Mahoney | Demoted to reserve roster |   |   | Before |
| 6/23/1965 | Bal | Johnson,D | Demoted to reserve roster |   |   | Before |
| 6/23/1965 | Bos | Moses | Demoted to reserve roster |   |   | Before |
| 6/23/1965 | ChA | Wills | Demoted to reserve roster |   |   | Before |
| 6/23/1965 | Det | Hamilton | Demoted to reserve roster |   |   | Before |
| 6/23/1965 | Det | Pena | Acquired via trade | KC |   | Before |
| 6/23/1965 | Det | Sullivan | Promoted to active roster |   |   | Before |
| 6/23/1965 | KC | Pena | Traded away | Det |   | Before |
| 6/23/1965 | KC | Rosario | Promoted to active roster |   |   | Before |
| 6/23/1965 | LA | Ferrara | Demoted to reserve roster |   |   | Before |
| 6/23/1965 | LA | LeJohn | Promoted to active roster |   |   | Before |
| 6/23/1965 | NYA | Repoz | Promoted to active roster |   |   | Before |
| 6/24/1965 | ChN | Hoeft | Promoted to active roster |   |   | Before |
| 6/24/1965 | Mil | Eilers | Promoted to active roster |   |   | Before |
| 6/24/1965 | SF | Hundley | Promoted to active roster |   |   | Before |
| 6/26/1965 | ChA | Pizarro | Placed on disabled list |   |   | Before |
| 6/29/1965 | ChN | Warner | Demoted to reserve roster |   |   | Before |
| 6/29/1965 | Det | Regan | Demoted to reserve roster |   |   | Before |
| 6/29/1965 | Mil | Alomar | Demoted to reserve roster |   |   | Before |
| 6/29/1965 | Min | Kostro | Demoted to reserve roster |   |   | Before |
| 6/29/1965 | NYA | Clarke | Demoted to reserve roster |   |   | After |
| 7/1/1965 | Cal | Gotay | Demoted to reserve roster |   |   | Before |
| 7/1/1965 | Cal | Koppe | Promoted to active roster |   |   | Before |
| 7/1/1965 | Cal | Latman | Demoted to reserve roster |   |   | Before |
| 7/1/1965 | Cal | Sukla | Promoted to active roster |   |   | Before |
| 7/1/1965 | ChN | Bright | Demoted to reserve roster |   |   | Before |
| 7/1/1965 | ChN | Faul | Promoted to active roster |   |   | Before |
| 7/1/1965 | Pit | Butters | Activated from disabled list |   |   | Before |
| 7/1/1965 | Pit | Pagan | Demoted to reserve roster |   |   | Before |
| 7/1/1965 | Pit | Smith | Promoted to active roster |   |   | Before |
| 7/1/1965 | SF | Burda | Promoted to active roster |   |   | Before |
| 7/1/1965 | SF | Hundley | Demoted to reserve roster |   |   | Before |
| 7/2/1965 | Cle | Hedlund | Placed on disabled list |   |   | Before |
| 7/2/1965 | Hou | Lee | Demoted to reserve roster |   |   | Before |
| 7/3/1965 | NYN | Bethke | Demoted to reserve roster |   |   | Before |
| 7/3/1965 | NYN | Musgraves | Promoted to active roster |   |   | Before |
| 7/4/1965 | Bos | Heffner | Promoted to active roster |   |   | Before |
| 7/4/1965 | Bos | Lamabe | Demoted to reserve roster |   |   | Before |
| 7/5/1965 | Bal | Blair | Demoted to reserve roster |   |   | Before |
| 7/5/1965 | KC | Reynolds | Promoted to active roster |   |   | Before |
| 7/5/1965 | KC | Stahl | Demoted to reserve roster |   |   | Before |
| 7/5/1965 | Was | Chance | Demoted to reserve roster |   |   | Before |
| 7/5/1965 | Was | Nen | Promoted to active roster |   |   | Before |
| 7/6/1965 | Mil | Eilers | Demoted to reserve roster |   |   | Before |
| 7/6/1965 | NYA | Brenneman | Promoted to active roster |   |   | Before |
| 7/6/1965 | NYA | Mikkelsen | Promoted to active roster |   |   | Before |
| 7/6/1965 | NYA | Tiefenauer | Demoted to reserve roster |   |   | After |
| 7/7/1965 | Bos | Gosger | Promoted to active roster |   |   | Before |
| 7/7/1965 | Bos | Ryan | Demoted to reserve roster |   |   | Before |
| 7/7/1965 | NYA | Stafford | Placed on disabled list |   |   | Before |
| 7/8/1965 | ChA | Hicks | Promoted to active roster |   |   | Before |
| 7/8/1965 | ChA | Lary | Acquired via trade | NYA |   | Before |
| 7/8/1965 | NYN | Lary | Traded away | ChA |   | Before |
| 7/8/1965 | NYN | Richardson | Promoted to active roster |   |   | Before |
| 7/8/1965 | Was | Moore | Demoted to reserve roster |   |   | Before |
| 7/10/1965 | Hou | Thomas | Acquired via trade | Phi |   | Before |
| 7/10/1965 | Phi | Phillips | Promoted to active roster |   |   | Before |
| 7/10/1965 | Phi | Thomas | Traded away | Hou |   | Before |
| 7/11/1965 | Pit | Pagan | Promoted to active roster |   |   | Before |
| 7/11/1965 | Pit | Smith | Demoted to reserve roster |   |   | Before |
| 7/12/1965 | Pit | Butters | Released |   |   | Before |
| 7/13/1965 | Cal | Egan | Placed on disabled list |   |   | Before |
| 7/13/1965 | Cal | Piersall | Activated from disabled list |   |   | Before |
| 7/17/1965 | Min | Boswell | Placed on disabled list |   |   | Before |
| 7/18/1965 | Min | Allen | Demoted to reserve roster |   |   | Before |
| 7/18/1965 | Min | Fosnow | Demoted to reserve roster |   |   | Before |
| 7/18/1965 | Min | Quilici | Promoted to active roster |   |   | Before |
| 7/18/1965 | Min | Roggenburk | Promoted to active roster |   |   | Before |
| 7/18/1965 | Min | Siebler | Promoted to active roster |   |   | Before |
| 7/18/1965 | NYA | Cullen | Promoted to active roster |   |   | Before |
| 7/19/1965 | LA | Brewer | Activated from disabled list |   |   | Before |
| 7/19/1965 | LA | Valle | Demoted to reserve roster |   |   | Before |
| 7/19/1965 | NYN | Spahn | Released |   |   | Before |
| 7/20/1965 | Cal | Smith,BG | Demoted to reserve roster |   |   | Before |
| 7/20/1965 | KC | Aker | Promoted to active roster |   |   | Before |
| 7/20/1965 | KC | Hickman | Demoted to reserve roster |   |   | Before |
| 7/20/1965 | KC | Mathews | Demoted to reserve roster |   |   | Before |
| 7/20/1965 | KC | Tartabull | Promoted to active roster |   |   | Before |
| 7/21/1965 | Cle | Moran | Promoted to active roster |   |   | Before |
| 7/21/1965 | Det | Moore | Demoted to reserve roster |   |   | Before |
| 7/21/1965 | Det | Navarro | Promoted to active roster |   |   | Before |
| 7/21/1965 | Mil | Dillard | Promoted to active roster |   |   | Before |
| 7/21/1965 | Mil | Gonder | Acquired via trade | NYN |   | Before |
| 7/21/1965 | Mil | Kolb | Traded away | NYN |   | Before |
| 7/21/1965 | NYN | Gonder | Traded away | Mil |   | Before |
| 7/21/1965 | NYN | Kolb | Acquired via trade | Mil |   | Before |
| 7/22/1965 | SF | Hiatt | Demoted to reserve roster |   |   | Before |
| 7/22/1965 | SF | Spahn | Signed |   |   | Before |
| 7/23/1965 | Cin | Arrigo | Demoted to reserve roster |   |   | Before |
| 7/23/1965 | Cin | Davidson | Promoted to active roster |   |   | Before |
| 7/23/1965 | Cin | Locke | Promoted to active roster |   |   | Before |
| 7/24/1965 | Bal | Warwick | Acquired via trade | StL |   | Before |
| 7/24/1965 | StL | Savage | Promoted to active roster |   |   | Before |
| 7/24/1965 | StL | Warwick | Traded away | Bal |   | Before |
| 7/26/1965 | ChA | Schaffer | Traded away | NYN |   | Before |
| 7/26/1965 | Hou | Kasko | Activated from disabled list |   |   | Before |
| 7/26/1965 | NYA | Brenneman | Demoted to reserve roster |   |   | Before |
| 7/26/1965 | NYN | Schaffer | Acquired via trade | ChA |   | Before |
| 7/27/1965 | ChA | Pizarro | Activated from disabled list |   |   | Before |
| 7/28/1965 | Cin | Duffalo | Demoted to reserve roster |   |   | Before |
| 7/29/1965 | Min | Pascual | Placed on disabled list |   |   | Before |
| 7/30/1965 | ChN | Burton | Promoted to active roster |   |   | Before |
| 7/30/1965 | ChN | Hendley | Demoted to reserve roster |   |   | Before |
| 7/31/1965 | Bal | Roberts | Released |   |   | Before |
| 7/31/1965 | Hou | Fox | Released |   |   | Before |
| 8/1/1965 | Mil | Carroll | Promoted to active roster |   |   | Before |
| 8/1/1965 | Mil | Klimchock | Demoted to reserve roster |   |   | Before |
| 8/1/1965 | Min | Merritt | Promoted to active roster |   |   | Before |
| 8/1/1965 | StL | Buchek | Placed on disabled list |   |   | Before |
| 8/2/1965 | Cle | Hargan | Promoted to active roster |   |   | Before |
| 8/2/1965 | Cle | Spring | Demoted to reserve roster |   |   | Before |
| 8/2/1965 | StL | Javier | Activated from disabled list |   |   | Before |
| 8/4/1965 | ChA | Hicks | Demoted to reserve roster |   |   | After |
| 8/4/1965 | NYN | Bethke | Promoted to active roster |   |   | Before |
| 8/4/1965 | NYN | Napoleon | Demoted to reserve roster |   |   | Before |
| 8/5/1965 | Bal | Belanger | Promoted to active roster |   |   | Before |
| 8/5/1965 | Bal | Blair | Promoted to active roster |   |   | Before |
| 8/5/1965 | Bal | Bowens | Demoted to reserve roster |   |   | Before |
| 8/5/1965 | Mil | Cowan | Acquired via trade | NYN |   | Before |
| 8/5/1965 | Mil | Kelley | Demoted to reserve roster |   |   | After |
| 8/5/1965 | NYN | Cowan | Traded away | Mil |   | Before |
| 8/5/1965 | NYN | Sutherland | Promoted to active roster |   |   | Before |
| 8/6/1965 | Hou | MacKenzie | Demoted to reserve roster |   |   | Before |
| 8/6/1965 | Hou | Roberts | Signed |   |   | Before |
| 8/6/1965 | NYN | Bearnarth | Demoted to reserve roster |   |   | After |
| 8/8/1965 | NYA | Stafford | Activated from disabled list |   |   | Before |
| 8/8/1965 | NYN | Musgraves | Placed on disabled list |   |   | Before |
| 8/9/1965 | NYN | Moorhead | Promoted to active roster |   |   | Before |
| 8/9/1965 | StL | Ricketts | Promoted to active roster |   |   | Before |
| 8/9/1965 | StL | Schultz | Demoted to reserve roster |   |   | Before |
| 8/10/1965 | Min | Kosco | Promoted to active roster |   |   | Before |
| 8/10/1965 | NYA | Edwards | Demoted to reserve roster |   |   | Before |
| 8/11/1965 | Cal | Egan | Activated from disabled list |   |   | Before |
| 8/11/1965 | Cle | Tiefenauer | Acquired via trade | NYA |   | Before |
| 8/11/1965 | Cle | Weaver | Demoted to reserve roster |   |   | Before |
| 8/11/1965 | NYA | Tiefenauer | Traded away | Cle |   | Before |
| 8/13/1965 | Min | Siebler | Demoted to reserve roster |   |   | Before |
| 8/14/1965 | KC | Krausse | Promoted to active roster |   |   | Before |
| 8/15/1965 | NYN | Kroll | Demoted to reserve roster |   |   | Before |
| 8/16/1965 | Bal | Belanger | Demoted to reserve roster |   |   | Before |
| 8/16/1965 | Hou | Adlesh | Promoted to active roster |   |   | Before |
| 8/16/1965 | Min | Boswell | Activated from disabled list |   |   | Before |
| 8/16/1965 | Min | Roggenburk | Demoted to reserve roster |   |   | Before |
| 8/17/1965 | Cal | Coates | Promoted to active roster |   |   | After |
| 8/17/1965 | Cal | Sukla | Demoted to reserve roster |   |   | After |
| 8/18/1965 | Bal | Knowles | Promoted to active roster |   |   | Before |
| 8/18/1965 | Cal | McBride | Demoted to reserve roster |   |   | Before |
| 8/18/1965 | Cal | Sanford | Acquired via trade | SF |   | Before |
| 8/18/1965 | Mil | Eilers | Waived | NYN |   | Before |
| 8/18/1965 | NYN | Eilers | Claimed on waivers | Mil |   | Before |
| 8/18/1965 | SF | Burda | Demoted to reserve roster |   |   | Before |
| 8/18/1965 | SF | Cepeda | Activated from disabled list |   |   | Before |
| 8/18/1965 | SF | Fuentes | Promoted to active roster |   |   | Before |
| 8/18/1965 | SF | Sanford | Traded away | Cal |   | Before |
| 8/20/1965 | Hou | Triandos | Released |   |   | Before |
| 8/22/1965 | Det | McAuliffe | Placed on disabled list |   |   | Before |
| 8/23/1965 | ChA | Freese | Acquired via trade | Pit |   | Before |
| 8/23/1965 | Cin | Helms | Promoted to active roster |   |   | Before |
| 8/23/1965 | Det | Smith | Promoted to active roster |   |   | Before |
| 8/23/1965 | Pit | Freese | Traded away | ChA |   | Before |
| 8/23/1965 | SF | Marichal | Suspended |   |   | Before |
| 8/23/1965 | Was | Duckworth | Promoted to active roster |   |   | Before |
| 8/24/1965 | Pit | Face | Activated from disabled list |   |   | Before |
| 8/24/1965 | Was | Duren | Released |   |   | Before |
| 8/26/1965 | Cin | Ruiz | Placed on disabled list |   |   | Before |
| 8/31/1965 | Bal | Haddix | Traded away | Mil |   | Before |
| 8/31/1965 | Cle | Hedlund | Activated from disabled list |   |   | Before |
| 8/31/1965 | NYN | Collins | Activated from disabled list |   |   | Before |
| 9/1/1965 | ChN | Holtzman | Promoted to active roster |   |   | Before |
| 9/1/1965 | Cin | Arrigo | Promoted to active roster |   |   | Before |
| 9/1/1965 | Cin | May | Promoted to active roster |   |   | Before |
| 9/1/1965 | Cin | Osteen | Promoted to active roster |   |   | Before |
| 9/1/1965 | Cle | Scheinblum | Promoted to active roster |   |   | Before |
| 9/1/1965 | Cle | Weaver | Promoted to active roster |   |   | Before |
| 9/1/1965 | Det | Moore | Promoted to active roster |   |   | Before |
| 9/1/1965 | Det | Regan | Promoted to active roster |   |   | Before |
| 9/1/1965 | Det | Roman | Promoted to active roster |   |   | Before |
| 9/1/1965 | Det | Stanley | Promoted to active roster |   |   | Before |
| 9/1/1965 | Hou | Thomas | Traded away | Mil |   | Before |
| 9/1/1965 | KC | Joyce | Promoted to active roster |   |   | Before |
| 9/1/1965 | KC | Odom | Promoted to active roster |   |   | Before |
| 9/1/1965 | LA | Ferrara | Promoted to active roster |   |   | Before |
| 9/1/1965 | Mil | Alomar | Promoted to active roster |   |   | Before |
| 9/1/1965 | Mil | Olivo | Promoted to active roster |   |   | Before |
| 9/1/1965 | Mil | Thomas | Acquired via trade | Hou |   | Before |
| 9/1/1965 | Min | Allen | Promoted to active roster |   |   | Before |
| 9/1/1965 | Min | Cimino | Promoted to active roster |   |   | Before |
| 9/1/1965 | Min | Pascual | Activated from disabled list |   |   | Before |
| 9/1/1965 | Min | Reese | Promoted to active roster |   |   | Before |
| 9/1/1965 | Min | Tovar | Promoted to active roster |   |   | Before |
| 9/1/1965 | NYN | Bearnarth | Promoted to active roster |   |   | Before |
| 9/1/1965 | NYN | Gardner | Promoted to active roster |   |   | Before |
| 9/1/1965 | NYN | Goossen | Promoted to active roster |   |   | Before |
| 9/1/1965 | NYN | Harrelson | Promoted to active roster |   |   | Before |
| 9/1/1965 | NYN | Jones | Promoted to active roster |   |   | Before |
| 9/1/1965 | NYN | Kroll | Promoted to active roster |   |   | Before |
| 9/1/1965 | NYN | Napoleon | Promoted to active roster |   |   | Before |
| 9/1/1965 | NYN | Ribant | Promoted to active roster |   |   | Before |
| 9/1/1965 | NYN | Selma | Promoted to active roster |   |   | Before |
| 9/1/1965 | NYN | Willey | Promoted to active roster |   |   | Before |
| 9/1/1965 | Phi | Jackson | Promoted to active roster |   |   | Before |
| 9/1/1965 | Phi | Sorrell | Promoted to active roster |   |   | Before |
| 9/1/1965 | Pit | Oliver | Promoted to active roster |   |   | Before |
| 9/1/1965 | Pit | Walker | Promoted to active roster |   |   | Before |
| 9/1/1965 | SF | Estelle | Promoted to active roster |   |   | Before |
| 9/1/1965 | SF | Hiatt | Promoted to active roster |   |   | Before |
| 9/1/1965 | SF | Marichal | Reinstated |   |   | Before |
| 9/1/1965 | SF | Priddy | Promoted to active roster |   |   | Before |
| 9/1/1965 | StL | Buchek | Activated from disabled list |   |   | Before |
| 9/1/1965 | Was | Chance | Promoted to active roster |   |   | Before |
| 9/1/1965 | Was | Cottier | Promoted to active roster |   |   | Before |
| 9/1/1965 | Was | Craig | Promoted to active roster |   |   | Before |
| 9/2/1965 | Bal | Belanger | Promoted to active roster |   |   | Before |
| 9/2/1965 | Bal | Bertaina | Promoted to active roster |   |   | Before |
| 9/2/1965 | Bal | Bowens | Promoted to active roster |   |   | Before |
| 9/2/1965 | Bal | Etchebarren | Promoted to active roster |   |   | Before |
| 9/2/1965 | Bal | Johnson,D | Promoted to active roster |   |   | Before |
| 9/2/1965 | ChN | Hendley | Promoted to active roster |   |   | Before |
| 9/2/1965 | NYA | Clarke | Promoted to active roster |   |   | Before |
| 9/2/1965 | NYA | Edwards | Promoted to active roster |   |   | Before |
| 9/2/1965 | NYA | Lopez,A | Promoted to active roster |   |   | Before |
| 9/2/1965 | NYA | Moore | Promoted to active roster |   |   | Before |
| 9/2/1965 | Pit | May | Promoted to active roster |   |   | Before |
| 9/2/1965 | StL | Aust | Promoted to active roster |   |   | Before |
| 9/2/1965 | StL | Francis | Promoted to active roster |   |   | Before |
| 9/2/1965 | StL | Jaster | Promoted to active roster |   |   | Before |
| 9/2/1965 | StL | Kernek | Promoted to active roster |   |   | Before |
| 9/2/1965 | StL | Schultz | Promoted to active roster |   |   | Before |
| 9/2/1965 | StL | Tolan | Promoted to active roster |   |   | Before |
| 9/3/1965 | Cin | Zanni | Promoted to active roster |   |   | Before |
| 9/3/1965 | Min | Siebler | Promoted to active roster |   |   | Before |
| 9/3/1965 | Min | Uhlaender | Promoted to active roster |   |   | Before |
| 9/4/1965 | ChN | Browne | Promoted to active roster |   |   | Before |
| 9/4/1965 | ChN | Hartenstein | Promoted to active roster |   |   | Before |
| 9/4/1965 | ChN | Young | Promoted to active roster |   |   | Before |
| 9/5/1965 | Cin | Duffalo | Promoted to active roster |   |   | Before |
| 9/6/1965 | Cin | Queen | Promoted to active roster |   |   | Before |
| 9/6/1965 | Det | Hiller | Promoted to active roster |   |   | Before |
| 9/6/1965 | Det | Redmond | Promoted to active roster |   |   | Before |
| 9/6/1965 | Min | Roggenburk | Promoted to active roster |   |   | Before |
| 9/6/1965 | NYA | Beck | Promoted to active roster |   |   | Before |
| 9/6/1965 | NYA | Jurewicz | Promoted to active roster |   |   | Before |
| 9/6/1965 | NYA | White | Promoted to active roster |   |   | Before |
| 9/7/1965 | Cal | Dees | Promoted to active roster |   |   | Before |
| 9/7/1965 | Cal | Hernandez | Promoted to active roster |   |   | Before |
| 9/7/1965 | Cal | Kirkpatrick | Promoted to active roster |   |   | Before |
| 9/7/1965 | Cal | Latman | Promoted to active roster |   |   | Before |
| 9/7/1965 | Cal | McBride | Promoted to active roster |   |   | Before |
| 9/7/1965 | Cal | McGlothlin | Promoted to active roster |   |   | Before |
| 9/7/1965 | Cal | Reichardt | Promoted to active roster |   |   | Before |
| 9/7/1965 | Cal | Simpson | Promoted to active roster |   |   | Before |
| 9/7/1965 | Cal | Smith,BG | Promoted to active roster |   |   | Before |
| 9/7/1965 | Cal | Sukla | Promoted to active roster |   |   | Before |
| 9/7/1965 | ChA | Agee | Promoted to active roster |   |   | Before |
| 9/7/1965 | ChA | Heath | Promoted to active roster |   |   | Before |
| 9/7/1965 | ChA | Hicks | Promoted to active roster |   |   | Before |
| 9/7/1965 | ChA | Josephson | Promoted to active roster |   |   | Before |
| 9/7/1965 | ChA | Kenworthy | Promoted to active roster |   |   | Before |
| 9/7/1965 | ChA | Staehle | Promoted to active roster |   |   | Before |
| 9/7/1965 | ChA | Voss | Promoted to active roster |   |   | Before |
| 9/7/1965 | ChN | Boccabella | Promoted to active roster |   |   | Before |
| 9/7/1965 | ChN | Bright | Promoted to active roster |   |   | Before |
| 9/7/1965 | ChN | Campbell | Promoted to active roster |   |   | Before |
| 9/7/1965 | ChN | Pena | Promoted to active roster |   |   | Before |
| 9/7/1965 | Cle | Banks | Promoted to active roster |   |   | Before |
| 9/7/1965 | Cle | Carreon | Promoted to active roster |   |   | Before |
| 9/7/1965 | Cle | Davis | Promoted to active roster |   |   | Before |
| 9/7/1965 | Cle | Kelley | Promoted to active roster |   |   | Before |
| 9/7/1965 | Det | Holtgrave | Promoted to active roster |   |   | Before |
| 9/7/1965 | Hou | Hoffman | Promoted to active roster |   |   | Before |
| 9/7/1965 | Hou | Jones | Promoted to active roster |   |   | Before |
| 9/7/1965 | Hou | Miller | Promoted to active roster |   |   | Before |
| 9/7/1965 | Hou | Ray | Promoted to active roster |   |   | Before |
| 9/7/1965 | Hou | Von Hoff | Promoted to active roster |   |   | Before |
| 9/7/1965 | KC | Drabowsky | Promoted to active roster |   |   | Before |
| 9/7/1965 | KC | Hickman | Promoted to active roster |   |   | Before |
| 9/7/1965 | KC | Monteagudo | Promoted to active roster |   |   | Before |
| 9/7/1965 | KC | Schwartz | Promoted to active roster |   |   | Before |
| 9/7/1965 | KC | Stahl | Promoted to active roster |   |   | Before |
| 9/7/1965 | KC | Tompkins | Promoted to active roster |   |   | Before |
| 9/7/1965 | LA | Griffith | Promoted to active roster |   |   | Before |
| 9/7/1965 | LA | Oliver | Promoted to active roster |   |   | Before |
| 9/7/1965 | LA | Singer | Promoted to active roster |   |   | Before |
| 9/7/1965 | LA | Valle | Promoted to active roster |   |   | Before |
| 9/7/1965 | LA | Werhas | Promoted to active roster |   |   | Before |
| 9/7/1965 | Mil | Aaron,T | Promoted to active roster |   |   | Before |
| 9/7/1965 | Mil | Beauchamp | Promoted to active roster |   |   | Before |
| 9/7/1965 | Mil | Kelley | Promoted to active roster |   |   | Before |
| 9/7/1965 | Phi | Jenkins | Promoted to active roster |   |   | Before |
| 9/7/1965 | Phi | Steevens | Promoted to active roster |   |   | Before |
| 9/7/1965 | SF | Barton | Promoted to active roster |   |   | Before |
| 9/7/1965 | SF | Brown | Promoted to active roster |   |   | Before |
| 9/7/1965 | SF | Burda | Promoted to active roster |   |   | Before |
| 9/7/1965 | SF | Hands | Promoted to active roster |   |   | Before |
| 9/7/1965 | SF | Hundley | Promoted to active roster |   |   | Before |
| 9/7/1965 | Was | Alyea | Promoted to active roster |   |   | Before |
| 9/7/1965 | Was | Casanova | Promoted to active roster |   |   | Before |
| 9/7/1965 | Was | Coleman | Promoted to active roster |   |   | Before |
| 9/7/1965 | Was | French | Promoted to active roster |   |   | Before |
| 9/7/1965 | Was | Hannan | Promoted to active roster |   |   | Before |
| 9/7/1965 | Was | McCabe | Promoted to active roster |   |   | Before |
| 9/7/1965 | Was | Moore | Promoted to active roster |   |   | Before |
| 9/7/1965 | Was | Valentine | Promoted to active roster |   |   | Before |
| 9/8/1965 | Bal | Barnowski | Promoted to active roster |   |   | Before |
| 9/8/1965 | Cal | Clinton | Waived | KC |   | Before |
| 9/8/1965 | KC | Clinton | Claimed on waivers | Cal |   | Before |
| 9/8/1965 | NYA | Murcer | Promoted to active roster |   |   | Before |
| 9/9/1965 | Cle | Clinton | Claimed on waivers | KC |   | Before |
| 9/9/1965 | KC | Clinton | Waived | Cle |   | Before |
| 9/9/1965 | KC | Blanchard | Traded away | Mil |   | After |
| 9/9/1965 | Mil | Blanchard | Acquired via trade | KC |   | Before |
| 9/10/1965 | KC | Paige | Promoted to active roster |   |   | Before |
| 9/11/1965 | ChN | Bright | Demoted to reserve roster |   |   | Before |
| 9/11/1965 | ChN | Burton | Demoted to reserve roster |   |   | Before |
| 9/11/1965 | Hou | Bateman | Promoted to active roster |   |   | Before |
| 9/11/1965 | Hou | Harrison | Promoted to active roster |   |   | Before |
| 9/11/1965 | Hou | Jackson | Promoted to active roster |   |   | Before |
| 9/11/1965 | Hou | Sembera | Promoted to active roster |   |   | Before |
| 9/11/1965 | Hou | White | Promoted to active roster |   |   | Before |
| 9/11/1965 | Hou | Zachary | Promoted to active roster |   |   | Before |
| 9/14/1965 | Hou | Lamabe | Promoted to active roster |   |   | Before |
| 9/14/1965 | KC | Lindblad | Promoted to active roster |   |   | Before |
| 9/15/1965 | Bos | Moses | Promoted to active roster |   |   | Before |
| 9/15/1965 | Bos | Ryan | Promoted to active roster |   |   | Before |
| 9/15/1965 | Cle | Gagliano | Promoted to active roster |   |   | Before |
| 9/15/1965 | Pit | Spriggs | Promoted to active roster |   |   | Before |
| 9/21/1965 | Cle | Gonzalez | Suspended |   |   | Before |
| 9/21/1965 | NYN | Musgraves | Activated from disabled list |   |   | Before |
| 9/22/1965 | Det | Marentette | Promoted to active roster |   |   | Before |
| 9/22/1965 | Hou | Arlich | Promoted to active roster |   |   | Before |
| 9/23/1965 | LA | Davis,T | Activated from disabled list |   |   | Before |
| 9/28/1965 | Mil | Aaron,T | Demoted to reserve roster |   |   | Before |
| 10/3/1965 | Cin | James | Demoted to reserve roster |   |   | Before |
| 10/3/1965 | Cin | Locke | Demoted to reserve roster |   |   | Before |
| 10/3/1965 | Cin | May | Demoted to reserve roster |   |   | Before |
| 10/3/1965 | Cin | Osteen | Demoted to reserve roster |   |   | Before |
| 10/3/1965 | Cin | Queen | Demoted to reserve roster |   |   | Before |
| 10/3/1965 | Cin | Zanni | Demoted to reserve roster |   |   | Before |
| 10/4/1965 | Cin | Ruiz | Activated from disabled list |   |   | Before |
| 10/4/1965 | Min | Allen | Demoted to reserve roster |   |   | Before |
| 10/4/1965 | Min | Cimino | Demoted to reserve roster |   |   | Before |
| 10/4/1965 | Min | Kosco | Demoted to reserve roster |   |   | Before |
| 10/4/1965 | Min | Reese | Demoted to reserve roster |   |   | Before |
| 10/4/1965 | Min | Sevcik | Demoted to reserve roster |   |   | Before |
| 10/4/1965 | Min | Siebler | Demoted to reserve roster |   |   | Before |
| 10/4/1965 | Min | Tovar | Demoted to reserve roster |   |   | Before |
| 10/4/1965 | Min | Uhlaender | Demoted to reserve roster |   |   | Before |